Heat resistant amino acid biosynthesis enzyme gene of high temperature resistant Corynebacterium

摘要

An L-amino acid biosynthetic enzyme derived from the cholinergic bacterium thermogenicity, preferably between the known gene sequences of the various microorganisms corresponding to the genes encoding enzymes that function at higher temperatures than the cholinebacterium glutamate As a set of primers designed on the basis of the amino acid level conserved regions, PCR was carried out as a template for PCR of primers obtained by amplified fragments To the limer, select a clone containing the desired DNA fragment from the plasmid library of the chromosome DNA of the cholinebacterium thermoaminogenicity.