首页> 外国专利> NEW PRIMER SET FOR DETECTING NUCLEIC ACID MARKER DERIVED FROM CHROMOSOME OF BARLEY ON WHEAT BACKGROUND AND UTILIZATION THEREOF

NEW PRIMER SET FOR DETECTING NUCLEIC ACID MARKER DERIVED FROM CHROMOSOME OF BARLEY ON WHEAT BACKGROUND AND UTILIZATION THEREOF

机译:一种用于检测小麦背景下大麦染色体的核酸标记的新底漆及其利用

摘要

PROBLEM TO BE SOLVED: To provide new primer sets for detecting a nucleic acid marker derived from a chromosome of barley on a wheat background, and to provide a method for utilizing the same.;SOLUTION: The new primer sets are obtained by originally designing primer sets for six nucleic markers positioned on a long arm of 1H chromosome of the barley and screening such primer sets as exhibiting difference (polymorphism) in amplification result between a case in which a genome DNA of the barley is used as a template and another case in which a genome DNA of the wheat is used as the template. Each of the obtained new primer sets comprising six sets is useful for deciding whether the 1H chromosome of the barley is introduced into the wheat or not. Further, some of the primer sets are useful for judging whether a hybrid progeny between the wheat and the barley has fertility or not.;COPYRIGHT: (C)2003,JPO
机译:解决的问题:提供新的引物对,用于检测小麦背景上大麦染色体的核酸标记,并提供利用该方法的方法。在大麦的1H染色体的长臂上放置了六个核酸标记,并筛选出以大麦的基因组DNA为模板的情况与在另一例中在扩增结果上表现出差异(多态性)的引物对。小麦的基因组DNA用作模板。所获得的每组新的包含六组的新引物可用于确定是否将大麦的1H染色体引入小麦。此外,一些引物组可用于判断小麦和大麦之间的杂交后代是否具有可育性。版权所有:(C)2003,JPO

著录项

  • 公开/公告号JP2003111593A

    专利类型

  • 公开/公告日2003-04-15

    原文格式PDF

  • 申请/专利权人 JAPAN SCIENCE & TECHNOLOGY CORP;

    申请/专利号JP20010309184

  • 发明设计人 TAKEDA MAKOTO;TAKEDA KAZUYOSHI;

    申请日2001-10-04

  • 分类号C12N15/09;C12Q1/68;G01N33/50;

  • 国家 JP

  • 入库时间 2022-08-22 00:17:26

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