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DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
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机译:用于丝状真菌中多肽分泌的DNA序列,载体和融合多肽
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摘要
The invention includes novel fusion nucleic acids encoding fusion polypeptides which when expressed in a filamentous fungus result in the expression of fusion polypeptides. The fusion nucleic acids comprise four nucleic acids which encode a fusion polypeptide comprising first, second, third and fourth amino acid sequences. The first nucleic acid encodes a signal polypeptide functional as a secretory sequence in a first filamentous fungus. The second nucleic acid encodes a secreted polypeptide or functional portion thereof which is normally secreted from the same filamentous fungus or a second filamentous fungus. The third nucleic acid encodes a cleavable linker while the fourth nucleic acid comprises at least two nucleic acids encoding desired polypeptides.
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