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Method for separation and purification of nucleic acid and unit for separation and purification of nucleic acid

机译:分离和纯化核酸的方法以及分离和纯化核酸的单元

摘要

An object of the present invention is to provide a method for separation and purification of nucleic acid, which has excellent separating properties, high washing efficiency, and easy processability, which uses mass-producible solid phases with substantially uniform separating capacities, and wherein procedures can be automated, and a unit for separation and purification of nucleic acid suitable for implementing this method. The object of the present invention was attained by a method for separation and purification of nucleic acid comprising the steps of adsorbing nucleic acid onto a solid phase and desorbing the nucleic acid from the solid phase, wherein the solid phase comprises a base material in which a graft polymer chain having a hydrophilic group is bonded onto the surface; and a unit for separation and purification of nucleic acid which comprises, in a container having at least two openings, a solid phase of a base material in which a graft polymer chain having a hydrophilic group is bonded onto its surface.
机译:本发明的一个目的是提供一种用于核酸分离和纯化的方法,其具有优异的分离性能,高洗涤效率和容易的可加工性,其使用具有基本均匀的分离能力的可大量生产的固相,并且其中步骤可以自动化,并且是分离和纯化核酸的单元,适合于实施该方法。本发明的目的是通过一种分离和纯化核酸的方法来实现的,该方法包括将核酸吸附到固相上并从固相上解吸核酸的步骤,其中固相包括基础材料,其中具有亲水性基团的接枝聚合物链键合在表面上。核酸分离和纯化单元,其在具有至少两个开口的容器中包括基材的固相,在该固相中,具有亲水基团的接枝聚合物链键合在其表面上。

著录项

  • 公开/公告号US2003135039A1

    专利类型

  • 公开/公告日2003-07-17

    原文格式PDF

  • 申请/专利权人 MAKINO YOSHIHIKO;MORI TOSHIHIRO;

    申请/专利号US20020305110

  • 发明设计人 YOSHIHIKO MAKINO;TOSHIHIRO MORI;

    申请日2002-11-27

  • 分类号C07H21/04;B01D15/08;

  • 国家 US

  • 入库时间 2022-08-22 00:10:17

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