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Arrangement for detecting biomolecular reactions and interactions
Arrangement for detecting biomolecular reactions and interactions
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机译:检测生物分子反应和相互作用的装置
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摘要
An arrangement for the detection of biomolecular reactions and interactions using the RS screening method and comprises a specimen plate or microtiter plate (PP or MTP), a white light source with subsequently arranged illumination beam path, a splitter plate on which the PP or the MTP is set for generating a detection beam path, and optical means for imaging the measurement beam path and reference beam path on a spatially resolving detector array of a CCD camera which is connected with an evaluating unit or with a computer for determining measurement values. The arrangement particularly provides optical imaging elements for imaging the measurement beam path and reference beam path at different positions on the detector array of the CCD camera simultaneously and without overlap, a monochromator which comprises a plurality of interference filters and which is arranged subsequent to the light source in the illumination beam path; a component assembly which collimates the illumination beam path, is formed of optical elements and is arranged between the monochromator and the splitter plate; and specimen plate or microtiter plate, and an optical imaging element for complete spatal separation of the measurement beam path and reference beam path in, or in the vicinity of, an intermediate inage plane or aperture diaphragm plane.
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