Process for the preparation of recombinant proteins in E. coli by high cell density fermentation

摘要

The invention relates to a fed-batch fermentation method with particular host-vector systems of E. coli for effective formation of recombinant proteins, in particular recombinant antibody molecules, preferably antibody fragments such as mini antibodies. In the conditions given the E. coli cells can grow at the maximum specific growth rate to very high cell densities. When recombinant product formation starts, only the product formed acts in a limiting manner on growth. Growth limitation by substrates or metabolic by-products does not occur. Consequently, large amounts of recombinant proteins can be produced in relation to space and time.