HIGH THROUGHPUT PURIFICATION, CHARACTERIZATION AND IDENTIFICATION OF RECOMBINANT PROTEINS

摘要

The invention provides high throughput assays for rapidly and simultaneously purifying, quantifying, determining the solubility profile, determining the purity and identifying a plurality of recombinant proteins. The method comprises affinity protein purification; proteolytic digestion and analysis of the protein fragments by mass spectrometry in multi-well microfiber plates. The recombinant proteins are linked to a tag-peptide through a proteolytic enzyme recognition site.