FILAMENT-RECRUITING FLUORESCENT PROTEINS FOR THE ANALYSIS AND IDENTIFICATION OF PROTEIN-PROTEIN-INTERACTIONS

摘要

The invention relates to bait-fusion proteins/complexes with aggregating properties, for example filament-forming properties, and prey-proteins which enable a fluorescent technical detection after recruiting in bait-structures. The combined use of said peptides enables the 'optical' analysis of already known protein-protein-interaction, including the identification of modulators of said interaction e.g. low molecular non-proteinogenic substances, in addition to the identification of novel interaction partners from a prey-polypeptide-gene library.