-2 -2 EXPRESSION SYSTEM OF HUMAN TIMP-2 PROTEIN AND RECOMBINANT HUMAN TIMP-2 PROTEIN

摘要

PURPOSE: Provided are an expression system of human TIMP-2(tissue inhibitor of metalloproteinase-2) protein and a recombinant human TIMP-2 protein. The recombinant human TIMP-2 protein can be useful for inhibition of cancer metastasis and treatment of diseases associated with angiogenesis. CONSTITUTION: A transformed strain, which expresses a gene encoding the human TIMP-2 protein having the nucleotide sequence of SEQ ID NO: 1, is produced by transforming a wild type or mutant type Yarrowia lipolytica with a vector containing the TIMP-2 gene of SEQ ID NO: 1, wherein the transformed strain is YLTXIn, YLTYIn or YLTZIn; and the transformed strain is YLTXIn-pmr1(KCTC 18087P). The recombinant human TIMP-2 protein, which is expressed from the transformed strain capable of expressing the TIMP-2 gene of SEQ ID NO: 1, has the amino acid sequence of SEQ ID NO: 7.