METHOD FOR PREPARING NOVEL GRASS BY INTERBREEDING BETWEEN DISTANT RELATIVE GRASSES USING GENETIC FACTOR CROSSING-OVER

摘要

Purpose: providing a method, has cold resistance and hot tolerance to prepare new grass for preparing a new grass for passing through interbreeding between the distant relative's grass exchanged using genetic factor. Construction: a kind of method is included the following steps: for preparing between the distant relative's grass exchanged using genetic factor by a new grass of interbreeding: making the pressure of the 10-2 millimetress of mercury of warm season zoysia grass crossing and 3 days at -5 DEG C or under conditions of less temperature with dead season English grass; The poroma of interbreeding grass is obtained in a MS medium culture interbreeding grass embryos at 25 DEG C; Cultivate the poroma in the interbreeding grass for the regenerated free MS media of a hormone of poroma.