PURPOSE: A preparation method of lipid soluble antioxidant microencapsulated with polysaccharides and proteins, microencapsules containing the lipid soluble antioxidant produced by the method, and a medical, nutritious or sitological composition containing the microencapsulated lipid soluble antioxidant prepared by the method are provided. CONSTITUTION: The preparation method of lipid soluble antioxidant microencapsulated with polysaccharides and proteins comprises a step (a) of mixing food emulsifier with an aqueous solution in which polysaccharides and proteins are dispersed to a certain concentration, heating and sterilizing the mixture, and preparing a transparent aqueous solution by maintaining temperature of the heated and sterilized mixture to 40 to 60 deg.C; a step (b) of preparing an emulsion by mixing the solution using homogenizer after adding fatty solution in which lipid soluble antioxidant is dissolved to the aqueous solution prepared in the step (a); a step (c) of preparing suspension containing microcapsules by spraying the emulsion prepared in the step (b) onto water having a temperature of 5 to 25 deg.C at high pressure; and a step (d) of improving stability of coating material by storing the suspension prepared in the step (c) at a low temperature range of 0 to 4 deg.C, wherein the lipid soluble antioxidant is selected from the group consisting of retinol or derivatives thereof, -tocopherol, -tocopherol, -tocopherol, and mixture thereof.
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