New Han Fei-viral gene delivery system and a method for manufacturing the same

摘要

PURPOSE: A method of delivering genes into various uterine cancer cells using a virus-like particle (VLP) is provided to improve delivering efficiency by binding a gene to VLP then treating with polyoxyethylene-polyoxypropylene block copolymer. CONSTITUTION: The method comprises the following steps of: producing VLP from recombinant protein obtained by infecting Papillomavirus capsid antigen or other virus capsid antigen into insect cells; producing VLP-PL conjugate by conjugating polylysine with VLP; obtaining VLP-PL-pCMV beta-gal plasmid complex, VLP-PL-pSV2CAT plasmid complex or VLP-PL-pCMV-p53 plasmid complex by linking plasmid pCMV beta-gal, pSV2CAT, pCMV-p53, respectively; treating the complex polyoxyethylene-polyoxypropylene block copolymer before transfecting into uterine cancer cell line.