New test for the ATPase.

摘要

A method for detecting and measuring orthophosphate (PI) radiolabeled in an aqueous reaction mixture, comprising the steps of: a. adding a solution of molybdate to said reaction mixture under acidic conditions to form a complex fosfomolidabto; and b. contacting said phosphomolybdate complex with a hydrophobic surface of a scintillant; whereby binding of phosphomolybdate to said surface provides enough proximity to one for the radiolabeled phosphate to induce measurable scintillation of the scintillant, which correlates with the amount of said orthophosphate.