Detection kit, assay plate to be used therein, detection method, evaluation method, polyclonal antibody of frog vitellogenin and procss for producing the same

摘要

A primary antibody recognizing vitellogenin is immobilized on the surface of a well preliminarily formed on a plate. Then a sample obtained from a subject exposed to an environment is injected into the well and reacted. Subsequently, a secondary antibody, which is labeled with an enzyme and recognizes the above vitellogenin, is injected and reacted. By injecting a color-development agent, a coloration reaction is carried out and the coloration level is measured. Based on the coloration level, the amount of the vitellogenin is calculated and thus the environment is evaluated based on the amount of the vitellogenin.