ASSAY FOR THE DETECTION AND QUANTITATION OF HEMIMETHYLATION

摘要

Abstract There is disclosed a method to measure the fraction of DNA molecules that ishemi-methylated at a specific CpG dinucleotide in a particular DNA sequence, in a pool of DNA molecules having mixed DNA methylation states at said CpG dinucleotide. There is also disclosed methods to calculate the rates of de novo-, and maintenance-methylation per cell division that occur at said CpG dinucleotide. Additionally, there is disclosed methylation kits for use in measuring and quantitating hemi-methylation, and for calculating the rates of de novo-, and maintenance-methylation.