Extraction and purification method of constituents from stem of Lonicera japonica Thunb. and the constituents of injection drug

摘要

PURPOSE: Provided is a method of extracting and purifying the effective active components from the aerial parts of Lonicera japonica which involves enhanced solubility and blood safety with excellent painkilling and anti-inflammatory effects. CONSTITUTION: The method of extracting and purifying the effective active components from the aerial parts of Lonicera japonica comprises the steps of: reflux-extracting the aerial parts of Lonicera japonica with water, and filtering it; adding the same amount of water-saturated short chain alcohol to isolate a layer; vacuum concentrating the alcohol layer to form a powder extract; purifying the extract with polyamide resin or polyvinylpyrrolidone resin; and purifying it with octadecylsilane. The short chain alcohol is butyl alcohol or propyl alcohol. The effective active components are sweroside and loganin.