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Image and characterization of the focal field of a lens by spatial autocorrelation

机译:通过空间自相关对镜头焦场进行成像和表征

摘要

Method and system for realising a correlation, at least in space, of the focal fields of at least two electromagnetic beams at the focus of an optical focusing element, wherein a specific spatial distribution in amplitude, phase and polarisation of a focal field combined from the focal fields is generated and a physical response to the generated focal field in a sample placed at the focus is measured. A one- or multi-dimensional spatial shift in the focal field of at leat one of the at least two beams is introduced with respect to the focal field of others of the at least two beams. The relative phase of the beams is controlled by a (periodic) variable optical path length for at least one of the beams. The physical response, as a consequence of a physical process in the sample or as a consequence of scattering of the focal field at the sample, is detected, optionally with the aid of a specific collection function which is a combined image of a specific space/time distribution of the physical response at the focus of the focusing element. Microscopic imaging with increased lateral and/or axial resolution is possible when the physical response of a sample subjected to the above-mentioned focal fields is detected by means of a collection function, which may or may not be specific. In addition, the quantitative structure in space and time, related to the point spread function, of the focal field of the focusing element on and alongside the optical axis thereof can be determined in terms of amplitude, phase or polarisation.
机译:用于在光学聚焦元件的焦点处实现至少两个电磁束的聚焦场的至少在空间上的相关性的方法和系统,其中,聚焦场的振幅,相位和偏振的特定空间分布与产生焦点场,并测量放置在焦点处的样品中对产生的焦点场的物理响应。相对于至少两个光束中的其他光束的至少一个,引入至少两个光束中的至少一个光束的焦点中的一维或多维空间偏移。光束的相对相位由光束中至少一个的(周期性)可变光程长度控制。由于样品中的物理过程或由于样品处的聚焦场的散射而导致的物理响应被检测到,可选地借助于特定的收集功能,该特定的收集功能是特定空间/聚焦元件聚焦时物理响应的时间分布。当通过收集功能检测到经受上述聚焦场的样品的物理响应时,可能具有增加的横向和/或轴向分辨率的显微成像。另外,可以根据振幅,相位或偏振来确定聚焦元件在其光轴上和旁边的,与点扩展函数有关的时空定量结构。

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