REAGENT COMPOSITION FOR ANALYZING RESIDUAL LEUCOCYTE IN BLOOD BANK PRODUCT HAVING REDUCED NUMBER OF LEUCOCYTE

摘要

PROBLEM TO BE SOLVED: To provide a reagent for highly accurately and precisely analyzing and counting residual leucocytes.;SOLUTION: The method for counting and analyzing the residual leucocytes in a sample of a blood product selected from a group of plasma, erythrocytes, and platelets and having a reduced number of leucocytes includes a process (a) for forming a suspension of the blood product having a reduced number of leucocytes by bringing the blood product having a reduced number of leucocytes into contact with a sufficient quantity of a dissolving reagent, which contains both a low molar concentration of a buffer agent (i) and a nonionic surface-active agent (ii); a process (b) for incubating the suspension of the product having a reduced number of leucocytes and the dissolving reagent at temperatures sufficient for selectively dissolving platelets and erythrocytes without damaging the leucocytes over a sufficient period of time; a process (c) for bringing the leucocytes of the dissolved blood product into contact with pixels appropriate for dyeing the leucocytes; and a process (d) for measuring the number of the dyed leucocytes.;COPYRIGHT: (C)2005,JPO&NCIPI