DNA ENCODING HUMAN α AND β SUBUNITS OF NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR, CELLS TRANSFORMED THEREWITH, AND RECOMBINANT CELL LINE EXPRESSING A HUMAN α AND β SUBUNIT OF NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR

摘要

Isolated nucleic acid molecules, i.e., DNA or RNA encoding human neuronal nicotinic acetylcholine receptor alpha and beta subunits, mammalian and amphibian cells containing said DNA, methods for producing α and β subunits and recombinant (i.e., isolated or substantially pure) α subunits and β subunits are provided. In addition, cells expressing various multimeric combinations of subunits (i.e., α₁, α₂ α₃ α₄ α₅ α₆ and/or α₇ in combination with at least one of an α and β subunit are also provided. A recombinant, non-human cell line expressing the human α₇ subunit of nAChR is disclosed.