A METHOD FOR ACHIEVING SITE SPECIFIC INTEGRATION OF A DESIRED DNA AT A TARGET SITE IN A MAMMALIAN CELL VIA HOMOLOGOUS RECOMBINATION AND VECTORS FOR REALIZING THIS METHOD

摘要

A method for achieving site specific integration of a desired DNA at a target site in a mammalian cell via homologous recombination is described. This method provides for the reproducible selection of cell lines wherein a desired DNA is integrated at a predetermined transcripcionally active site previously marked with a marker plasmid. The method is particularly suitable for the production of mammalian cell lines which secrete mammalian proteins at high levels, in particular immunoglobulins. Vectors and vector combinations for use in the subject cloning method are also provided.