EPITOPES OF THE PRE-M / M PROTEIN OF DENGUE VIRUS, SYNTHETIC PEPTIDES.

摘要

THIS INVENTION IS ABOUT FIVE SYNTHETIC PEPTIDES BELONGING TO THE PRE - M / M PROTEIN OF VIRUS DENGUE - 2 CORRESPONDING TO THE AMINO ACID SEQUENCES 3-31-, 45-67, 57-92, 69-93 AND 103-124. THE IMMUNE RESPONSE TO THE PEPTIDES WAS EVALUATED IN MOUSES. RECOMBINANT FUSION PROTEINS THAT INCLUDE PRE - M / M REGIONS ARE ALSO BUILT. THE PRESENCE OF EPITHOPES FOR MOUSE AND HUMAN CELLS B IN THE PEPTIDES OF THE PRE - M / M PROTEIN OF VIRUS DENGUE IS CHECKED. PEPTIDES 3-31 AND 103-124 INDUJED NEUTRALIZING ANTIBODIES AGAINST THE FOUR SEROTYPES OF VIRUS DENGUE. LINFOPROLIFERATION STUDIES WITH PEPTIDES 57-92 AND 3-31 SHOWN CROSSED RECOGNITION OF PEPTIDE CELLS - SPECIFIC TO VIRUSES. MOUSES CHALLENGED WITH VIRUS DENGUE - 2 SHOWED PROTECTION Induced BY PEPTIDES 3-31, 57-92 AND 69-93. THEREFORE, THE PRESENCE OF SEQUENTIAL EPITHOPES IN THE PRE - M / M PROTEIN OF VIRUS DENGUE - 2 AND THE POSSIBLE RELEVANCE OF THEMSELVES IN THE IMMUNECONTRA RESPONSE SAID FLAVIVIRUS IS SHOWN.