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GENERATION OF RECOMBINANT ADENO-ASSOCIATED VIRAL VECTORS BY A COMPLETE ADENOVIRUS-MEDIATED APPROACH
GENERATION OF RECOMBINANT ADENO-ASSOCIATED VIRAL VECTORS BY A COMPLETE ADENOVIRUS-MEDIATED APPROACH
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机译:完整的腺病毒介导的方法产生重组腺相关病毒
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摘要
A complete virus-mediated system for recombinant AAV production including: (a) a first recombinant adenovirus encoding one or more AAV REP78/68 polypeptides and one or more viral capsid polypeptides; (b) a second recombinant adenovirus encoding a gene of interest and including AAV inverted terminal repeats, wherein the AAV inverted terminal repeats flank the gene of interest; (c) viral helper functions; and (d) a host cell transduced with the first recombinant adenovirus, the second recombinant adenovirus, and the viral helper functions.
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