Method for culturing Langerhans islets cells

摘要

A method for removing fibroblasts growing from the surface of in vitro proliferated Langerhans islets, comprising: (1) providing a plurality of proliferated islets having fibroblasts growing therewith and collected from a gel proliferation matrix; (2) adding an enzyme to the collected islets and to any gel adhering to the surface of the collected islets and incubating to provide an incubated product; and (3) aspirating the incubated product to cause the gel acted on by the enzyme to be removed from the islets, thereby exposing the fibroblasts to the force being created during the aspiration causing the fibroblasts to become separated from the surface of the islets to prepare fibroblast-free islets.