AN IMPROVED METHOD FOR PRODUCTION OF PORCINE CLONE EMBRYOS VIA SOMATIC CELL NUCLEAR TRANSFER

摘要

The present invention relates to an improved method for mass production of porcine embryos cloned via somatic cell nuclear transfer. In detail, oocytes were obtained from slaughterhouse-derived ovaries and then matured in vitro. Upon completion of in vitro maturation (IVM), the oocytes were enucleated and individually fused with somatic cells by electric stimulation. The reconstructed eggs were activated by secondary electric stimulation and then cultured in vitro. This invention is to establish optimal conditions to activate reconstructed oocytes and to enhance viability of nuclear transfer (NT) embryos, eventually by which result in viable cloned embryos and cloned animals. Therefore, the present invention of somatic cell nuclear transfer in the pig can be applied to multiply elite pigs and to develop transgenic pigs for xenotransplantation or as a disease model.