EXTRACT FROM PLANT somatic embryos, methods of preparation and use of an extract, a diagnostic system based on the EXTRACT AND ITS APPLICATION

摘要

1. extract from plant somatic embryos derived from in vitro u0443u0441u043bu043eu0432u0438u00a0u0445 u043au0443u043bu044cu0442u0438u0432u0438u0440u043eu0432u0430u043du0438u00a0, u0434u043bu00a0 u0431u0435u0441u043au043bu0435u0442u043eu0447u043du044bu0445 systems u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438 and / or u0441u043eu043fu0440u00a0u0436u0435u043du043du043eu0439 transcription - u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438.;2. extract for 1, where the u0441u043eu043cu0430u0442u0438u0447u0435u0441u043au0438u043cu0438 somatic embryos u00a0u0432u043bu00a0u044eu0442u0441u00a0 wheat germ.;3. extract for 1, where the u0441u043eu043cu0430u0442u0438u0447u0435u0441u043au0438u043cu0438 embryos u00a0u0432u043bu00a0u044eu0442u0441u00a0 somatic germ u00a0u0447u043cu0435u043du00a0.;4. extract for 1, where the u0441u043eu043cu0430u0442u0438u0447u0435u0441u043au0438u043cu0438 embryos u00a0u0432u043bu00a0u044eu0442u0441u00a0 somatic germ of corn.;5. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 extracts from plant somatic embryos on any of the u043fu043f.1 - 4 u0434u043bu00a0 u0431u0435u0441u043au043bu0435u0442u043eu0447u043du044bu0445 systems u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438 and / or u0441u043eu043fu0440u00a0u0436u0435u043du043du043eu0439 transcription - u0442u0440u0430u043du0441u043bu00a0u0446 s, including:;(a). a primary material of somatic tissues.;(b). u0441u043eu043cu0430u0442u0438u0447u0435u0441u043au043eu0433u043e embryogenesis induction.;(c). preparation of extract from somatic embryos.;6. way to p.5, further comprising induction u0441u043eu0441u0442u043eu00a0u043du0438u00a0 u043fu043eu043au043eu00a0 have u0441u0444u043eu0440u043cu0438u0440u043eu0432u0430u0432u0448u0438u0445u0441u00a0 somatic embryos.;7. method for u043fu043f.5 - 6, where u0434u043bu00a0 induction u0441u043eu0441u0442u043eu00a0u043du0438u00a0 u043fu043eu043au043eu00a0 using plant hormone selected from the group consisting of u0430u0431u0441u0446u0438u0437u043eu0432u0443u044e acid (aba) and its analogs.;8. method for u043fu043f.5 - 7, where u0434u043bu00a0 induction u043fu043eu043au043eu00a0 use ava in concentration of about 1 mg / l, which u0434u043eu0431u0430u0432u043bu00a0u044eu0442 in a permissive environment in approximately 15 days after the beginning of the mi products embryogenesis.;9. way to p.5, where at the stage of (a) the primary material derived from somatic tissues of wheat.;10. way to p.5, where at the stage of (a) the primary material derived from somatic tissues u00a0u0447u043cu0435u043du00a0.;11. way to p.5, where at the stage of (a) the primary material derived from somatic tissues of maize.;12. way to p.5, where at the stage (a) as the primary material used immature u0437u0438u0433u043eu0442u0438u0447u0435u0441u043au0438u0435 germ.;13. way on clause 5, when necessary, further comprising the step of induction between the primary u043au0430u043bu043bu0443u0441u0430 stage u043fu043eu043bu0443u0447u0435u043du0438u00a0 primary material from somatic tissue (a) and (b) u0441u043eu043cu0430u0442u0438u0447u0435u0441u043au043eu0433u043e induction stage of embryogenesis.;14. way to p.5, where in the stage (b) use artificial nutrient medium consisting of mineral salts, organic additives of different composition and concentration (sa u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442u044b hara, amino acids, protein, vitamins, u043eu0441u043cu043eu0440u0435u0433u0443u043bu00a0u0442u043eu0440u044b et al.), a plant hormone, as well as the agar or artificial or natural analogues.;15. way on p.14 where as sugar use glucose.;16. way on p.14 where as the sugar used u043cu0430u043bu044cu0442u043eu0437u0443.;17. way on p.14 where as sugar using sucrose.;18. a way to question 14, where u0434u043bu00a0 induction, embryogenesis using plant hormone selected from the group consisting of u0430u0443u043au0441u0438u043du043eu0432, 2.4 - u0434u0438u0445u043bu043eu0440u0444u0435u043du043eu043au0441u0438u0443u043au0441u0443u0441u043du0443u044e acid (2,4 d), 2,4,5 u0442u0440u0438u0445u043bu043eu0440u0444u0435u043d u043eu043au0441u0438u0443u043au0441u0443u0441u043du0443u044e acid (2,4,5-t), 3.6 - u0434u0438u0445u043bu043eu0440 - - yes, the anise one! good acid (Dicamba), r - u0445u043bu043eu0440u0444u0435u043du043eu043au0441u0438u0443u043au0441u0443u0441u043du0443u044e acid (cpa), and u043du0430u0444u0442u0438u043b - acetic acid (NAA), and / or a group of u0446u0438u0442u043eu043au0438u043du0438u043du043eu0432, switching consistent u043au0438u043du0435u0442u0438u043d and 6 u0431u0435u043du0437u0438u043bu0430u043cu0438u043du043eu043fu0443u0440u0438u043d (wa).;19. way on p.14, where as the main u0438u043du0434u0443u043au0442u043eu0440u0430 embryogenesis using 2,4 - d.;20. a way to question 14, where u0434u043bu00a0 u0443u0432u0435u043bu0438u0447u0435u043du0438u00a0 embryogenesis efficiency further using plant hormone selected from the group consisting of cytokinin and gibberellin.;21. method for u0434u043bu00a0 p.20, where u0443u0432u0435u043bu0438u0447u0435u043du0438u00a0 efficiency using Daminozide embryogenesis.;22. a way to question 14, where, as a u043eu0441u043cu043eu0440u0435u0433u0443u043bu00a0u0442u043eu0440u0430 using polyethylene glycol 6000 (peg 6000);23. way to p.5, where somatic germ in the cultivation of cells on the stage (b) u0444u043eu0440u043cu0438u0440u0443u044eu0442u0441u00a0 directly.;24. way to p.5, where somatic germ in the cultivation of cells on the stage (b) u0444u043eu0440u043cu0438u0440u0443u044eu0442u0441u00a0 through an intermediate stage u043eu0431u0440u0430u0437u043eu0432u0430u043du0438u00a0 u044du043cu0431u0440u0438u043eu0433u0435u043du043du043eu0433u043e u043au0430u043bu043bu0443u0441u0430.;25. extract the way on any of the u043fu043f.5 - 24.;26. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 extracts from plant somatic embryos on u043fu043f.1 - 4 u0434u043bu00a0 u0431u0435u0441u043au043bu0435u0442u043eu0447u043du044bu0445 systems u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438 and / or u0441u043eu043fu0440u00a0u0436u0435u043du043du043eu0439 transcription - u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438 that next:;(a). a primary material of somatic tissues.;(b). a high u044du043cu0431u0440u0438u043eu0433u0435u043du043du043eu0433u043e u043au0430u043bu043bu0443u0441u0430;;(c). a u044du043cu0431u0440u0438u043eu0433u0435u043du043du043eu0439 u0441u0443u0441u043fu0435u043du0437u0438u043eu043du043du043eu0439 culture;;(d). induction of secondary u0441u043eu043cu0430u0442u0438u0447u0435u0441u043au043eu0433u043e embryogenesis;;(e). preparation of extract from somatic embryos.;27. way on p.26, where at the stage of (a) the primary material derived from somatic tissues of wheat.;28. way on p.26, where at the stage of (a) the primary material derived from somatic tissues u00a0u0447u043cu0435u043du00a0.;29. way on p.26, where at the stage of (a) the primary material derived from somatic tissues of maize.;30. way on p.26, where at the stage (a) as the primary material used immature u0437u0438u0433u043eu0442u0438u0447u0435u0441u043au0438u0435 germ.;31. method for obtaining high u044du043cu0431u0440u0438u043eu0433u0435u043du043du043eu0433u043e p.26, where u043au0430u043bu043bu0443u0441u0430 under (b) use artificial nutrient medium consisting of mineral salts, organic d u043eu0431u0430u0432u043au0438 different composition and concentration (sugars, amino acids, u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442u044b proteins, vitamins, etc.), plant hormone, as well as the agar or artificial or natural and taxes.;32. method for p.31 where as sugar use glucose.;33. how to use u043cu0430u043bu044cu0442u043eu0437u0443 p.31 where as sugar.;34. method for p.31 where as sugar using sucrose.;35. method for p.31, where the plant hormone to choose from u0430u0443u043au0441u0438u043du0430 2.4 - u0434u0438u0445u043bu043eu0440u0444u0435u043du043eu043au0441u0438u0443u043au0441u0443u0441u043du043eu0439 acid (2,4 d) and / or a group of u0446u0438u0442u043eu043au0438u043du0438u043du043eu0432 comprising u043au0438u043du0435u0442u0438u043d and 6 u0431u0435u043du0437u0438u043bu0430u043cu0438u043du043e purine (ba).;36. method for obtaining u044du043cu0431u0440u0438u043eu0433u0435u043du043du043eu0439 p.26, where u0441u0443u0441u043fu0435u043du0437u0438u043eu043du043du043eu0439 culture under c) use artificial liquid nutrient medium comprising mineral salt organic additives of different composition and concentration (sugars, amino acids, vitamins, protein u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442u044b et al.), a plant hormone.;37. way on p.36, where as the sugar used u043cu0430u043bu044cu0442u043eu0437u0443.;38. way on p.36, where as the sugar using sucrose.;39. way on p.36, where the plant hormone u0430u0443u043au0441u0438u043du043eu0432 choose group consisting of 2,4 - u0434u0438u0445u043bu043eu0440u0444u0435u043du043eu043au0441u0438u0443u043au0441u0443u0441u043du0443u044e acid (2,4 d) and 2, 4, 5, trichlorophenoxyacetic acid (2) 4, 5 - t).;40. way on p.26, where secondary embryogenesis induction phase (d) use artificial nutrient medium consisting of mineral salts, organic additives the different composition and concentration (sugars, amino acids, u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442u044b proteins, vitamins, etc.), a plant hormone.;41. method for u0438u0441u043au0443u0441u0441u0442u0432u0435u043du043du0430u00a0 p.40, where u043fu0438u0442u0430u0442u0435u043bu044cu043du0430u00a0 environment additionally contains agar or artificial or natural analogues.;42. way on any of the u043fu043f.40 41, where as the sugar using sucrose.;43. way on any of the u043fu043f.40 41 where the chosen from the group consisting of plant hormone u0430u0443u043au0441u0438u043du043eu0432, 2.4 - u0434u0438u0445u043bu043eu0440u0444u0435u043du043eu043au0441u0438u0443u043au0441u0443u0441u043du0443u044e acid (2,4 d), 2,4,5 trichlorophenoxyacetic acid (2,4,5-t) and 3.6 - u0434u0438u0445u043bu043eu0440u043e - - yes, the anise one! good acid (Dicamba).;44. extract the way on any of the u043fu043f.26 - 43.;45. the application of the extract on any of the u043fu043f.1 - 4, 25,44 u0434u043bu00a0 u0431u0435u0441u043au043bu0435u0442u043eu0447u043du043eu0439 system u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438 and / or u0441u043eu043fu0440u00a0u0436u0435u043du043du043eu0439 transcription - u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438.;46. application on p.45 u0434u043bu00a0 u0431u0435u0441u043au043bu0435u0442u043eu0447u043du043eu0433u043e fusion u043eu043bu0438u0433u043eu043fu0435u043fu0442u0438u0434u043eu0432, polypeptides and proteins.;47. the application of the extract on any of the u043fu043f.1 - 4, 25,44 u0434u043bu00a0 production diagnostic system u0434u043bu00a0 u043eu0431u043du0430u0440u0443u0436u0435u043du0438u00a0 biologically active compounds with u043cu043eu0434u0443u043bu0438u0440u0443u044eu0449u0435u0439 and u043au0442u0438u0432u043du043eu0441u0442u044cu044e in the processes of transcription and / or u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438 and / or u0434u043bu00a0 screening such u0441u043eu0435u0434u0438u043du0435u043du0438u00a0.;48. u0434u0438u0430u0433u043du043eu0441u0442u0438u0447u0435u0441u043au0430u00a0 system on the basis of any of the u043fu043f.1 - 4, 25,44 u0434u043bu00a0 u043eu0431u043du0430u0440u0443u0436u0435u043du0438u00a0 biologically active compounds with u043cu043eu0434u0443u043bu0438u0440u0443u044eu0449u0435u0439 activity in percent u0435u0441u0441u044b transcription and / or u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438 and / or u0434u043bu00a0 screening such u0441u043eu0435u0434u0438u043du0435u043du0438u00a0.;49. the application of diagnostic system for u0434u043bu00a0 p.48 u043eu0431u043du0430u0440u0443u0436u0435u043du0438u00a0 biologically active compounds with u043cu043eu0434u0443u043bu0438u0440u0443u044eu0449u0435u0439 activity in the processes of transcription and / or u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438 and / or u0434u043bu00a0 screening such u0441u043eu0435u0434u0438u043du0435u043du0438u00a0.