METHOD FOR PREPARING RIBOFLAVIN, STRAINS BACILLUS SUBTILIS AS PRODUCERS OF RIBOFLAVIN (VARIANTS)

摘要

FIELD: biotechnology, microbiology, vitamins.;SUBSTANCE: method relates to a method for preparing riboflavin by culturing the microorganism Bacillus subtilis as a producer in the nutrient medium containing rib-operon from Bacillus amyloliquefaciens, or microorganism able for utilization of glycerophosphate as a single carbon source, or eliciting the resistance against inhibition of growth by glyoxylate, and extraction of riboflavin prepared. Invention uses the following strains as producers of riboflavin: B. subtilis GM51/pMX45, B. subtilis GM41/pMX45, B. subtilis GM44/pMX45. Invention provides preparing riboflavin of the high degree of effectiveness.;EFFECT: improved preparing method.;5 cl, 4 ex