PRIMER SET AND PROBE OLIGONUCLEOTIDE SPECIFIC FOR 10 KINDS OF BACTERIA ASSOCIATED WITH RESPIRATORY DISEASES

摘要

PPROBLEM TO BE SOLVED: To provide a primer set capable of specifically amplifying target sequences of ten kinds of bacteria associated with respiratory diseases and a probe oligonucleotide specific for each target sequence of the bacteria and not causing a cross reaction with other nucleic acids. PSOLUTION: Disclosed is a target sequence-amplifying primer set comprising an oligonucleotide having a fragment comprising continuous ten or more bases of a specific sequence and length of 10bp to 100bp, and an oligonucleotide having a fragment comprising continuous ten or more bases of another specific sequence and length of 10bp to 100bp, and specific for Bordetella pertussis, Chlamydophila pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumoniae, Moraxella catarrhalis, Mycoplasma pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae. Also disclosed is a probe oligonucleotide specific for each of the ten kinds of the bacteria. PCOPYRIGHT: (C)2006,JPO&NCIPI