Novel stationary phases for use in high-performance liquid chromatography

摘要

The invention provides novel materials for chromatography and chromatography columns. The invention provides a monofunctional silane or a mixture of two or three monofunctional silanes chemically bonded to a substrate, at least one monofunctional silane having at least one unsaturated hydrocarbon group, R′, covalently attached to the substrate, containing the silane structure is of form: Where R′ is independently selected from the group consisting of alkenyl, alkynyl, and phenyl, R″ is selected from the group consisting of alkyl, substitute alkyl, alkenyl, substitute alkenyl, alkynyl, and aryl, substitute aryl, R is selected from the group consisting of alkyl, substitute alkyl, alkenyl, substitute alkenyl, alkynyl, and aryl, substitute aryl, alkylamine, amide, ether, alcohol, carbamate, ester, an anion exchanger, and a cation exchange. Methods for manufacture and design of the columns are also provided and disclosed.