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Method for rapidly detecting quinolone-resistant Salmonella spp. and the probes and primers utilized therein

机译:快速检测耐喹诺酮沙门氏菌的方法。以及其中使用的探针和引物

摘要

The present invention relates to a method for rapidly detecting quinolone-resistant Salmonella spp. The invention also relates to the oligonucleotide primers and probes utilized in the method, which can differentiate mutant strains having one or two single point mutations in gyrA gene or single point mutation in parC gene from wild type strains, respectively. Said primers and probes can be effectively used for detection of nalidixic acid-resistant and/or ciprofloxacin-resistant Salmonella strains.
机译:快速检测耐喹诺酮类沙门氏菌的方法本发明涉及一种快速检测耐喹诺酮类沙门氏菌的方法。本发明还涉及在该方法中使用的寡核苷酸引物和探针,其可以将野生型菌株分别区分在gyrA基因中具有一个或两个单点突变或在parC基因中具有单点突变的突变菌株。所述引物和探针可以有效地用于耐耐萘啶酸和/或耐环丙沙星的沙门氏菌(I)沙门氏菌的检测。

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