Enzymatic conversion of racemic 2-arylpropionic acid esters to optically active S-2-arylpropionic acids using succinyl-cyclodextrins as the chiral selector

摘要

The present invention (R, S) -2 - propionic acid aryl ester racemate (racemic (R, S)-2-aryl-propionate ester) as a raw material of biocatalysts Lipase (lipase) or S. The atmospheres (esterase) to used in the optically active (S) - form 2-aryl propionic acid ((S)-two-arylpropionic acids) by biotransformation of the process in the chiral selectivity (chiral selectivity) is in succinyl-cyclodextrin (succinyl-cyclodextrins) was added by giving (S) - it relates to a process for preparing a 2-aryl propionic acid with high efficiency type. Adding a chiral agent selected succinyl-cyclodextrin is insoluble in raw materials (R, S) -2-aryl propionic acid with the optically active ester la from the racemate (S) - in combination with a water-soluble type and selectively formed jeopche PO and PO jeopche is thereby remarkably improved when the optical selectivity of biocatalysts lipase atmospheres or S. the reaction of the enzyme. Further addition of a succinyl-cyclodextrin is possible to significantly increase the solubility of the racemate la insoluble state of (R, S) -2- aryl propionic acid ester in an aqueous reaction adding a high concentration of the raw material. Thus it can be prepared according to methods (R, S) -2- ah reel acid ester la from the optically active semi-body (S) -2- aryl acid of the present invention to selectively high purity, high yield and high concentration. ; (S) - 2-aryl propionic acid type, (R, S) -2- aryl acid ester racemates, succinyl-cyclodextrin, lipase, an optically active isomer