Oligonucleotide for genotyping of Mycoplasma, microarray comprising the oligonucleotide, and method for detection of species using the microarray

摘要

The present invention relates to a method for detecting Mycoplasma and its related strains which are source of contamination of cell lines and biological products and human pathogenic. More particularly, the present invention relates to genus-specific and species-specific oligonucleotides for genotyping of Mycoplasma, Acholeplasm and Ureaplasma strains, microarray comprising the oligonucleotides, and method for detection of species using the microarray. As described above, the present invention provides a rapid and accurate assay method capable of simultaneously detecting many Mycoplasma and its related strains from a single sample using a microarray comprising novel oligonucleotides for detecting Mycoplasma and its related strains which are known as a source of contamination of cell lines and biological products and human pathogenic. Further, the present invention provides an objective and credible assay method capable of tracing a contamination source for preventing expansion of infective Mycoplasma and its related strains and controlling a contamination of Mycoplasma against biological products and stem cells or cord blood cells which are useful for gene therapy and cell therapy.