Recombinant plasmid DNA PCL1 and PCH1, WITH PROPERTIES encodes a polypeptide light and heavy chains of human antibodies against the virus Ebola, provide for the common biosynthesis of full-length human antibodies against the virus Ebola in mammalian cells, and the recombinant full-length antibody HUMAN CLASS IGG1, interacts with Ebola virus

摘要

1. u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du0430u00a0 u043fu043bu0430u0437u043cu0438u0434u043du0430u00a0 dna pCL1, u043au043eu0434u0438u0440u0443u044eu0449u0430u00a0 synthesis in mammalian cells u043fu043eu043bu0438u043fu0435u043fu0442u0438u0434u0430 with properties of light chain on the full-scale u043cu043eu043du043eu043au043bu043eu043du0430u043bu044cu043du043eu0433u043e u0447u0435u043bu043eu0432u0435u0447u0435u0441u043au043eu0433 the antibodies, cooperative with the ebola virus, the size of the 6108 sukhoy. and u043cu043eu043bu0435u043au0443u043bu00a0u0440u043du043eu0439 weighing 4.03 yeah, u0432u043au043bu044eu0447u0430u044eu0449u0430u00a0:;NheI / XhoI - vector fragment, plasmid pcDNA3.1 (+) (Invitrogen) of 5338 u043f.u043e. containing u0446u0438u0442u043eu043cu0435u0433u0430u043bu043eu0432u0438u0440u0443u0441u043du044bu0439 (cmv) promoter and enhancer transcription site u043fu043eu043bu0438u0430u0434u0435u043du0438u043bu0438u0440u043eu0432u0430u043du0438u00a0, uch u0430u0441u0442u043eu043a transcription termination BGH, gene - lactamase (bla) and the gene of resistance to the u043du0435u043eu043cu0438u0446u0438u043du0443;;NheI / XhoI - fragment of 770 u043f.u043e. containing artificial gene, encrypting fusion protein, in which the u0432u0430u0440u0438u0430u0431u0435u043bu044cu043du044bu0439 domain light chain u043eu0434u043du043eu0446u0435u043fu043eu0447u0435u0447u043du043eu0433u043e antibodies 4D1, u043bu0443u0447u0435u043du043du043eu0433u043e of combinatorial antibody libraries u043eu0434u043du043eu0446u0435u043fu043eu0447u0435u0447u043du044bu0445 u0444u0430u0433u043eu0432u043eu0439 man, connected with the u043au043eu043du0441u0442u0430u043du0442u043du044bu043c domain kappa chain igg, a u043du0443u043au043bu0435u043eu0442u0438u0434u043du0443u044e after u0434u043eu0432u0430u0442u0435u043bu044cu043du043eu0441u0442u044c contained in u0444u0438u0433.1;;genetic markers:;gene - lactamase (bla), u043eu043fu0440u0435u0434u0435u043bu00a0u044eu0449u0438u0439 sustainability u0442u0440u0430u043du0441u0444u043eu0440u043cu0438u0440u043eu0432u0430u043du043du044bu0445 u043fu043bu0430u0437u043cu0438u0434u043eu0439 pCL1 cells of bacteria to u0430u043cu043fu0438u0446u0438u043bu043bu0438u043du0443;;the gene of resistance to the u043du0435u043eu043cu0438u0446u0438u043du0443 u0434u043bu00a0 breeding u0442u0440u0430u043du0441u0444u0435u0446u0438u0440u043eu0432u0430u043du043du044bu0445 u043fu043bu0430u0437u043cu0438u0434u043eu0439 pCL1 cells of mammals.;unique restriction sites u0443u0437u043du0430u0432u0430u043du0438u00a0 u044du043du0434u043eu043du0443u043au043bu0435u0430u0437u0430u043cu0438 with the following coordinates: BglII - 12, NheI 895, XmaJI - 1294, XhoI - 1665.;2. u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du0430u00a0 u043fu043bu0430u0437u043cu0438u0434u043du0430u00a0 dna u0440u0441u043d1, u043au043eu0434u0438u0440u0443u044eu0449u0430u00a0 synthesis in mammalian cells u043fu043eu043bu0438u043fu0435u043fu0442u0438u0434u0430 with properties of u0442u00a0u0436u0435u043bu043eu0439 full-scale u043cu043eu043du043eu043au043bu043eu043du0430u043bu044cu043du043eu0433u043e u0447u0435u043bu043eu0432u0435u0447u0435u0441 chain one antibody, cooperative with the ebola virus, the size of the 6917 sukhoy. and u043cu043eu043bu0435u043au0443u043bu00a0u0440u043du043eu0439 weighs 4.57 yeah, u0432u043au043bu044eu0447u0430u044eu0449u0430u00a0:;NheI / XhoI - vector fragment of plasmid pcDNA3.1 (+) (Invitrogen) of 5338 u043f.u043e. containing cmv promoter and enhancer transcription termination site, the site u043fu043eu043bu0438u0430u0434u0435u043du0438u043bu0438u0440u043eu0432u0430u043du0438u00a0, tran u0441u043au0440u0438u043fu0446u0438u0438 BGH, gene - lactamase (bla) and the gene of resistance to the u043du0435u043eu043cu0438u0446u0438u043du0443;;NheI / XhoI - fragment of 1579 u043f.u043e. containing artificial gene, encrypting fusion protein, in which the u0432u0430u0440u0438u0430u0431u0435u043bu044cu043du044bu0439 domain u0442u00a0u0436u0435u043bu043eu0439 chain u043eu0434u043du043eu0446u0435u043fu043eu0447u0435u0447u043du043eu0433u043e antibodies 4D1, u0444u0430u0433u043eu0432u043eu0439 obtained from a combinatorial library u043eu0434u043du043eu0446u0435u043fu043eu0447u0435u0447u043du044bu0445 antibodies rights connected with u043au043eu043du0441u0442u0430u043du0442u043du044bu043cu0438 u0441u043d1 - u0441u043d2 - u0441u043d3 - domains IgG1 rights with u043du0443u043au043bu0435u043eu0442u0438u0434u043du0443u044e p u043eu0441u043bu0435u0434u043eu0432u0430u0442u0435u043bu044cu043du043eu0441u0442u044c contained in u0444u0438u0433.2;;genetic markers:;gene - lactamase (bla), u043eu043fu0440u0435u0434u0435u043bu00a0u044eu0449u0438u0439 sustainability u0442u0440u0430u043du0441u0444u043eu0440u043cu0438u0440u043eu0432u0430u043du043du044bu0445 u043fu043bu0430u0437u043cu0438u0434u043eu0439 u0440u0441u043d1 cells of bacteria to u0430u043cu043fu0438u0446u0438u043bu043bu0438u043du0443;;the gene of resistance to the u043du0435u043eu043cu0438u0446u0438u043du0443 u0434u043bu00a0 breeding u0442u0440u0430u043du0441u0444u0435u0446u0438u0440u043eu0432u0430u043du043du044bu0445 u043fu043bu0430u0437u043cu0438u0434u043eu0439 u0440u0441u043d1 cells of mammals.;unique restriction sites u0443u0437u043du0430u0432u0430u043du0438u00a0 u044du043du0434u043eu043du0443u043au043bu0435u0430u0437u0430u043cu0438 with the following coordinates: Bg1II - 12, NheI 895, ApaI - 1349, XhoI - 1326 and 2474.;3. u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du043eu0435 full sized human antibody class IgG1, u0432u0437u0430u0438u043cu043eu0434u0435u0439u0441u0442u0432u0443u044eu0449u0435u0435 with ebola virus, including u043fu043eu043bu0438u043fu0435u043fu0442u0438u0434u044b with the properties of light and u0442u00a0u0436u0435u043bu043eu0439 chains u0430u043du0442u0438u0442 eat man, the joint u0442u0440u0430u043du0441u0444u0435u043au0446u0438u0435u0439 cells of mammals u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du044bu043cu0438 u043fu043bu0430u0437u043cu0438u0434u043du044bu043cu0438 dna pCL1 and u0440u0441u043d1 having structure for u043fu043f.1 and 2.