METHOD FOR DETECTION OF MORPHOLOGICAL CHANGEABILITY AND STABILITY OF CORYNEFORMIC BACTERIUM CELLS

摘要

FIELD: microbiology, in particular method for detection of transformed coryneformic bacterium variants useful in production of specific preparations.;SUBSTANCE: coryneformic bacterium cells in 0.5-0.55 % sodium chloride solution are exposed with ultrasound (40-45 kHz, 83-85 mA, 5-30 min) and after that morphological changeability and stability thereof are determined. During exposing with 5 min interval change of cell morphology is analyzed, viable cells amount is determined, and presence of morphological changeability is evaluated based on formation of L-transformed cells being exposed for 5-15 min. Cell stability is evaluated based on reduction of viable cells amount: at 5-15 min exposition viability is evaluated as high, 16-25 min - as intermediate, and at 25-30 min exposition viability is absent.;EFFECT: method for detection of coryneformic bacterium changeability of improved effectiveness.;1 dwg, 2 tbl