METHODS FOR IDENTIFICATION OF P8 GENOTYPE ROTAVIRUS SUBTYPES (VARIANTS)

摘要

FIELD: molecular biology, medicine.;SUBSTANCE: invention relates to methods (variants) for identification of P[8] genotype rotavirus subtypes. Claimed method includes rotavirus RNA isolation followed by reverse transcription thereof. Further single-round polymerase chain reaction is carried out followed by analysis of obtained fragments in agarose gel. For identification of P[8]-1 subtype 5'-cca ttt att tga atc gtt a-3' primer is used, and for identification of P[8]-1 subtype 5'-cca ttt atc tga atc att a-3' primer is used. Presence of desired subtype is detected on the base of specific fragment with size of 410 n.p. Method of present invention is useful in detection of P[8] genotype rotavirus of various subtypes for monitoring of rotavirus strain circulation and investigation of their variability, abundance, etc.;EFFECT: simplified and accelerated method for strain differentiation.;2 cl, 2 ex