ANALYSIS OF FLUORESCENCE INTENSITY FOR DUPLEX AND TRIPLEX HYBRIDIZATION OF NUCLEIC ACIDS IN SOLUTION BY USING FLUORESCENT INTERCALATORS

摘要

FIELD: biochemistry, molecular biology.;SUBSTANCE: invention proposes two variants for analysis of binding a target comprising a polynucleotide sequence. In accordance with given variants a target is hybridized with a probe comprising a nucleic acid sequence or its analogue in the presence of intercalating fluorophore. A sample obtained in hybridization is irradiated by exciting radiation. As result of such treatment a fluorophore containing in a sample emits the fluorescent radiation. The degree of erroneous pairing a probe with a target or degree of the correspondence of probe and target are assayed by intensity if indicated radiation. Using the invention allows simplifying analysis of interaction between nucleic acids and/or analogues of nucleic acids and enhancing sensitivity and rate of the indicated process. Invention can be used for homogenous analysis of nucleic acids hybridization.;EFFECT: improved method of analysis.;29 cl, 13 dwg, 8 ex