Liquid medium for nerve cells, a method for its production and a method for the incubation of nerve cells with the use of the same

摘要

A liquid medium for nerve cells prepared by adding albumin to the culture supernatant of primary astroglia cells obtained by incubating the astroglia cells in a nutritional medium containing insulin and transferrin. By using the liquid medium, central nerve cells can be stably incubated. Thus, in low density incubation, synapses excellent in the elongation of axons can be quickly formed, while, in high density incubation, it is possible to provide cells forming a nervous network and being excellent in the stability over a prolonged storage.