MONOCLONAL ANTIBODIES OF MOUSE AND ITS APPLICATION IN THE SPECIFIC IDENTIFICATION OF LARVAS IN D OF MEJILLON MYTILUS GALLOPROVINCIALIS.

摘要

The larvae of many bivalve molluscs are very similar in morphology, requiring sophisticated and slow technology for precise identification. From Balb / C mice immunized with two-day (D) mussel larvae of the Mytilus galloprovincialis species, we have generated two hybridomas (HYB-M36.5 and HYB-M22.8) producing monoclonal antibodies from M36.5 and M22.8 mouse, respectively. Both antibodies, M36.5 and M22.8, specifically recognize the D larvae of Mytilus galloprovincialis mussel, but do not recognize the D larvae of other bivalves such as Ostrea edulis, Cerastoderma edule, Aequipecten opercularis, Tapes decussatus and Tapes philippinarum, so the present invention could be used in techniques that allow selective identification of mussel larvae Mytilus galloprovincialis.