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TROPHIC CONVERSION OF OBLIGATE PHOTOTROPHIC ALGAE THROUGH METABOLIC ENGINEERING

机译:通过代谢工程对难养光藻的营养转化

摘要

Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.
机译:大多数微藻是专性光合自养生物,其生长严格取决于光合作用产生的能量。在这项研究中表明,通过引入编码葡萄糖转运蛋白的基因,微藻藻角蛋白可以被设计为输入葡萄糖并在黑暗中生长。人和小球藻葡萄糖转运蛋白都促进了角果疟原虫对葡萄糖的吸收,使细胞能够代谢外源有机碳并在不依赖光的情况下壮成长。这是专一的光合自养生物通过代谢工程的第一个成功的营养转化,并且证明了通过单一基因的引入可以从根本上改变细胞营养的方法。由于用葡萄糖转运基因转化的菌株能够非光合生长,因此可以通过突变体的产生和表征将其用于光合作用的分析。最后,这项工作也代表了通过使用微生物发酵技术大规模开发专性光养藻类的重要进展,从而消除了光依赖性生长带来的显着限制。

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