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Method for detecting homo- or heterozygosity of an organism using labeled primers and RFLP
Method for detecting homo- or heterozygosity of an organism using labeled primers and RFLP
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机译:使用标记引物和RFLP检测生物体纯合或杂合性的方法
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摘要
The invention is a method for detecting whether an organism is homozygous or heterozygous in a target nucleotide. The method combines hybridization of two differently labelled probes A and B on either side of a restriction site, restriction endonuclease digestion, and detection of the labels in the resulting digest. If an organism is: homozygous then the labels will be present in the ratio 1:1 or 1:0; or if heterozygous then the labels will be present in the ratio 1:2. Either immobilized array technology or electrophoretic separation may be used.
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