The invention relates to a method of recording images of the bottom of a plate provided with at least one well (2) containing a culture medium (1) of microorganisms, characterized in that it comprises the steps of: i) forming an opacifying layer (5) covering the surface of the culture medium (1) so as to form a reading background, ii) taking pictures, at defined time intervals, of the bottom of the well (2). ) by means of an optical imaging device.
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