Isotopic code ionization enhancement reagent for the identification and quantification of the high-throughput protein using matrix assisted laser desorption ionization mass spectrometry (ICIER)

摘要

Useful arginine content cystein decoration chemical compound is offered to the MALDI-MS analysis of the protein. The isotope cord/code ionization reinforcement reagent (isotope-coded ionization enhancement reagents) (ICIER) with as for these chemical compounds which are named, additional sample operation it can offer ionization reinforcement, relative fixed quantity, and the addition database search condition in dying and MALDI-MS simultaneously. Further in details, ICIER increases the ionization efficiency of cystein content peptide with the addition of the guanidinoacetic functional group. ICIER, because of the hydrophily of ICIER increases also the entire hydrophily of these peptides, increases the recovery factor of these peptides in the operation and processing of the sample therefore like digestion in the gel or liquid kuromatogurahui. Lastly, light ICIER and heavy ICIER both combination, search condition of the addition database search which increases the reliability of the protein identification due to the relative fixed quantity, and peptide mass mapping of the protein with MALDI-MIS (the number of cystein inside all single peptide peaks residues) offers, the accurate expedient in order to obtain.