Analysis of the signature for identification of genes (GIS) for mapping transcribed

摘要

"Analysis of the signature for identification of genes (GIS) for mapping of transcripts. A method for the mapping of transcribed according to one implementation of the invention is described and combines efficiency, based on tags (SAGE and MPSS) short, with the precision of full length cDNA (flcdna) for a comprehensive characterization of transcritomas.This method is also called the analysis of gene identification signature (GIS).In this method, initially producing the ends 5 3 39 and 39 clones of cDNA full length inside a structure of ditag (double label), with the concatu00e2meros of ditag and subsequently placed in sequence in an efficient way, and finally mapped to the G Enoma for defining the gene structure.As a ditag of GIS represents the ends 5 3 39 and 39 of a transcribed it is more informative than tags of sage and MPSS. It is possible to obtain portions of segments between pairs of tags 3 5 39 and 39, including orientation, order and family of chromosomes for mapping efficiently transcribed and identification of the site of genes.Still, it uses a selection of suffixes tablets (CSA) to index the sequence of genomes in order to speed up the map and reduce the requirement of the memory of the computer.