Method, kit and primers for the identification of sequences nucleotu00ecdeos of Schistosoma mansoni by real time PCR

摘要

Method, kit and primers for the identification of sequences nucleotu00ecdeos of Schistosoma mansoni by real time PCR. The main objective of the present invention is the sensitive and specific detection of nucleotide sequence of Schistosoma mansoni by real-time quantitative PCR.The DNA of Schistosoma mansoni can be quantified in different samples, such as, but not limited to, serum, feces, snails and tissues. Indirectly, it provides an estimate of the parasite load of infection of vertebrate hosts and invertebrates, as well as allows to estimate the potential infectivity of foci of transmission of schistosomiasis.A first implementation of this invention refers to a method for the identification of nucleotide sequence of Schistosoma mansoni.Which is characterized by the following steps: (a) to collect the sample to be examined; (b) to extract the DNA of the sample obtained in (a); (c) to amplify the nucleotide sequence obtained in the step (b) by polymerase chain reaction (PCR) in real time; (d) to discriminate. To analyze the products Amplification of step (c) by appropriate techniques.A second concretization of the present invention relates to a kit for the identification of nucleotide sequence of Schistosoma mansoni, which employs the method above. A third concretization of the present invention relates to the initiator described in SEQ. The ID in the.1 (or sequences functionally equivalent to the same) to be employed in the above method or kit for the identification of nucleotide sequence of Schistosoma mansoni. A fourth concretization of the present invention is related to the initiator described in SEQ ID in.2 (or sequences functionally equivalent to the same) to be employed in the above method or kit for quantitative identification) nucleotide sequence of Schistosoma mansoni.