Strain of cyanobacterium Nostoc sp. Lukesovß 27/97 and isolation method of acetycholinesterase therefrom

摘要

The present invention relates the production strains cyanobacterium Nostoc sp. Lukešová 27/97, ​​the process of isolation of new biologically active substances from this strain and its own bioaktivnílátka possessing inhibitory activity against the enzyme acetylcholinesterase. Isolation process involves extracting biomass production strain methanol, evaporated to a crude methanolic extract to dryness, dissolving the residue in acetone and precipitating the active substance by addition of an excess of an aliphatic hydrocarbon. The active material was further chromatographed sequentially nasloupci polyamide, silica gel and using a semi-preparative reverse phase HPLC C.sub.18.n .. This yields an acetylcholinesterase inhibitor on the structural formula, 2,2'-bis [4,5-bis (4- hydroxybenzyl) -2- (4-hydroxyphenyl) cyclopent-4-ene-1,3-dione] with purity higher than 98%.