PROCESS AND MODIFIED MEDIA FOR PREPARING CALLUS- AND CELL SUSPENSION CULTURES OF HYPERICUM PERFORATUM L.

摘要

The invention relates to a process for preparing callus- and suspension cultures of Hypericum perforatum L. in modified media in order to produce increased amount of biomass and active ingredients. According to the invention the ratio of the macro- and microelements is altered in the known MS, N6 and B5 media, further the media are completed with EDTA-Na-Fe selecton iron and optionally with phytohormones. In such manner, continuously producing in vitro systems are obtained which produce the synergetic, biologically active components of H. perforatum L. simultaneously with the plant material. The invention also relates to the modified media.