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METAL MEDIATED FLUORESCENCE SUPERQUENCHING ASSAYS FOR DETECTION OF PHOSPHORYLATION AND DEPHOSPHORYLATION OF QUENCHER-LABELED SUBSTRATES
METAL MEDIATED FLUORESCENCE SUPERQUENCHING ASSAYS FOR DETECTION OF PHOSPHORYLATION AND DEPHOSPHORYLATION OF QUENCHER-LABELED SUBSTRATES
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机译:金属介导的荧光超猝灭测定法检测猝灭基团的磷酸化和去磷酸化
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摘要
A method includes contacting an analyte; a phosphorylatable or dephosphorylatable substrate bound to a quencher; and a sensor that includes at least one fluorescent polymer complexed to one or more metal species; to form a composition; and determining the fluorescence of the composition, if any; wherein when the substrate is phosphorylated, an association occurs between the substrate and the sensor such that fluorescent superquenching occurs between the fluorescent polymer and the quencher.
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