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Pseudotyping of retroviral vectors, methods for production and use thereof for targeted gene transfer and high-throughput screening
Pseudotyping of retroviral vectors, methods for production and use thereof for targeted gene transfer and high-throughput screening
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机译:逆转录病毒载体的假分型,其生产方法和用于靶向基因转移和高通量筛选的方法
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摘要
The invention relates to the pseudotyping of retroviral vectors with heterologous envelope proteins derived from the Paramyxoviridae family, genus Morbillivirus, and various uses of the resulting vector particles. The present invention is based on the unexpected and surprising finding that the incorporation of morbillivirus F and H proteins having truncated cytoplasmic tails into lentiviral vector particles, and the complex interaction of these two proteins during cellular fusion, allows for a superior and more effective transduction of cells. Moreover, these pseudotyped vector particles allow the targeted gene transfer into a given cell type of interest by modifying a mutated and truncated H protein with a single-chain antibody or ligand directed against a cell surface marker of the target cell.
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