DNA MARKER OF ADIPOCYTE-FATTY ACID BINDING PROTEIN GENE RELATED THE INTRAMUSCULAR FAT CONTENT IN BEEF CATTLE

摘要

A DNA marker is provided to determine an individual genetic trait of Korean native cattle by using genotypes of three independent loci of a FABP4(adipocyte-fatty acid binding protein) gene which has the significantly effect for carcass weight and marbling of the Korean native cattle, thereby being utilized for an early selection of a fatting beef and an accurate estimate of breeding values. A method for selecting a DNA marker of determining genetic traits comprises the steps of: (a) identifying a genotype of a linkage group detected from a Korean native cattle a fatty acid binding protein(FABP4) gene of SEQ ID : NO. 7 using the decoding of 17 SNP loci depicted at a Fig. 1; (b) after pre-denaturing the Korean native cattle FABP4 gene of SEQ ID : NO. 7 as a template using a PCR primer of SEQ ID : NOs. 1 to 6 at a temperature of 94 deg.C for 5 minutes, performing 35 times of a cycle where one minute annealing at a temperature of 58 deg.C and two-minute expanding at a temperature of 72 deg.C are done and then finally reacting it for 10 minutes at a temperature of 72 deg.C for PCR amplification; (c) after cleaving an amplification product obtained from the step(b) with an restriction enzyme such as Hpy188I, RsaI and NlaIII, subjecting it to an electrophoresis; and (d) obtaining a picture and an image of the electrophoresis product to determine an individual genotype through a restriction fragment length polymorphism analysis, a heteroduplex analysis, a single strand conformational polymorphism(SSCP) and a Taqman probe method. A DNA marker is selected by the method and constructed to recognize three loci of Hpy188I(C-G), RsaI(A-T) and NlaIII(A-G) described in the Fig.1 among variable regions of a FAB4 gene expressed in adipocyte.