METHOD FOR ASSAYING CELLULAR RESPIRATORY ACTIVITY AND KIT FOR THE METHOD, METHOD FOR SCREENING PROTEIN PRODUCTION-ENHANCING STRAIN AND KIT FOR THE METHOD

摘要

PROBLEM TO BE SOLVED: To ensure cellular respiratory activity, in an easy and swift fashion.;SOLUTION: This method for assaying cellular respiratory activity includes a process in which a cell to be assayed is brought into contact with 5-cyano-2,3-ditolyl-2H-tetrazolium chloride(CTC), and the resultant red fluorescent precipitate comprising CTC formazan in the assay sample is assayed in the well(s) of a plate. In this method, for example, this red fluorescent precipitate can be detected by measuring the 420 nm wavelength absorbance, alternatively, by measuring the 630-640 nm wavelength fluorescence which is excited by an exciting wavelength near 450 nm.;COPYRIGHT: (C)2009,JPO&INPIT