The curative medicine of the fusion protein, and the Hirotune Characteristic heaven smallpox which are recognized in the Hirotune Characteristic heaven smallpox patient self antibody, being the remedy instrument, and the fusion

摘要

PURPOSE: To obtain a fused protein capable of being recognized with an autoantibody of a pemphigus vulgaris patient by ligating the hinge part of the constant region of IgG to the C-terminal of the extracellular region of a pemphigus vulgaris antigen protein. CONSTITUTION: A fused protein is produced by ligating the hinge part of the constant region of IgG to the C-terminal of a protein which is represented by the sequence of the formula and which is the extracellular region (PVAg) of a pemphigus vulgaris antigen protein. When a gene coding the fused protein is transduced into an animal cell or an insect cell to express the fused protein, the expressed fused protein can accurately be discharged from the cell, because of using the amino acid sequence of the PVAg. Since the hinge part of the constant region of the IgG is ligated, the fused protein can stably be produced, and the high-dimensional structure of the PVAg is the same as that of the natural PVAg. Since the IgG is ligated to the cut part of the C-terminal side, the autoantibody can surely be recognized. Further, since the IgG is contained, the presence or absence of the expressron after transduced in the cell can easily be examined, and the purification of the fused protein after the expression is facilitated.